Question: Eqtl Analysis & Snps In Microarray Probes
1
gravatar for Darren J. Fitzpatrick
6.7 years ago by
Ireland/ United Kingdom
Darren J. Fitzpatrick1.1k wrote:

Hi,

I have a set of probes from a custom microarray. I have the probe coordinates as mapped to the most recent genome build.

I wish to quantify the amount of probes with SNPs in them. I am only interested in quantifying probes with SNPs in Caucasians with a frequency > 0.05.

I attempted mapping the probes to the dbSNP chromosome reports. However, these do not contain SNP frequency nor population information.

Does anyone have any suggestions?

Thanks!

eqtl snp microarray • 1.5k views
ADD COMMENTlink written 6.7 years ago by Darren J. Fitzpatrick1.1k
1
gravatar for Larry_Parnell
6.7 years ago by
Larry_Parnell16k
Boston, MA USA
Larry_Parnell16k wrote:

Many dbSNP entries do have minor allele frequency data for certain populations. If your SNPs of interest do not, perhaps you can use a tool like SNAP (Broad Institute) to find those SNPs that are in absolute (r=1.0) or high LD (linkage disequilibrium (r > 0.8). An alternative is to use HapMap to capture the minor allele frequency data you want.

ADD COMMENTlink written 6.7 years ago by Larry_Parnell16k

Hi Larry - As per your suggestion, I opted to use the HapMap data from the latest release. By mapping my expression probes to dbSNP irrespective of population, ~2/3 of my probes contained SNPs. By restricting to the CEU HapMap population ~4000 of the 36,000 had SNPs. Thanks.

ADD REPLYlink modified 6.7 years ago • written 6.7 years ago by Darren J. Fitzpatrick1.1k
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