designing primers from CIRI2 output
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3.3 years ago
gnt • 0

Hi all, I used CIRI2 algorithm for detection of circRNA from my RNA-Seq data. I got a result of junction reads and circRNA co-ordinates, I wish to validate these circular RNA by using RT-PCR. How can i design divergent primers from the CIRI2 output?

RNA-Seq circRNA validation • 557 views
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