I'm trying to use Hisat2 on WSL, and I used
-wdp founding location of indexs (hg19 downloaded), fastq file and output.sam.
Warning: Could not open read file "/mnt/c/Users/.../trimmed_out.fastq" for reading; skipping... Error: No input read files were valid (ERR): hisat2-align exited with value 1
What should I do? Thanks for your reply
Thanks, all things are OK. problem solved.