When I try and map my fastq files in STAR, I get the error:
EXITING: because of fatal INPUT file error: could not open read file: Day-30-R3_S3_L008_R1_001.fastq.gz SOLUTION: check that this file exists and has read permision
However, I have checked that both the name and the directory of this file is correct, so I do not see the issue? Could anyone help out? Many thanks!
STAR --runThreadN 4 --genomeDir /Users/mfaleeva/Desktop/Day30 --readFilesCommand gunzip -c --readFilesIn Day-30-R1_S1_L008_R1_001.fastq.gz,Day-30-R2_S2_L008_R1_001.fastq.gz,Day-30-R3_S3_L008_R1_001.fastq.gz Day-30-R1_S1_L008_R2_001.fastq.gz,Day-30-R2_S2_L008_R2_001.fastq.gz,Day-30-R3_S3_L008_R2_001.fastq.gz --outSAMattrRGline ID:Day30_R1 , ID:Day30_R2 , ID:Day30_R3