Pacbio RSII rna handling
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6 months ago

I have downloaded transcriptomes from NCBI. these were Pacbio (RSII) read. But i dont know what should be best strategy to get transcripts. should i go for assembly? Or for alignment? If i go for alignment then how about the denovo transcripts? I downloaded Stringtie2. But what to input in it didnt understand clearly. Can someone please give me some clue? Thanks

alignment • 144 views
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If these are isoseq reads then they should be full length (more or less) based on my understanding. Have you checked to see what kind of reads they are. Post an example accession if you want a second opinion.

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