Question: how treat FLAG 16 in ribosome footprint
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gravatar for dwc06631
6 weeks ago by
dwc066310
dwc066310 wrote:

hello. first, sorry for that I'm not good at this field. I aligned reads to reference, and now I'm finding each read's start location from start codon to draw ribosome footprint figure. My question is

  1. how to treat FLAG 16. I understand that they aligned to reverse strand, so they expressed in reverse complement forms in sam files. so if i have read1 that POS:25 and FLAG:16, is it correct to point read1 in 25 location from start codon in ribosome footpring? Just treat like other flag0 reads?

  2. why we have reverse strand sequence in reads?

alignment • 89 views
ADD COMMENTlink modified 6 weeks ago • written 6 weeks ago by dwc066310
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