MGISEQ-2000 adapter trimming
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3.2 years ago

Hello community!

I want to take advice regarding proceccing of raw data obtained from MGISEQ-2000. I have 100 PE RNA-seq data.

  1. I wonder to know is there any specific features in fastq proceccing comparing with Illumina?

  2. I specially want to ask about trimming of adapters. I found sequences below for trimming.

Fwd AAGTCGGAGGCCAAGCGGTCTTAGGAAGACAA

Rev AAGTCGGATCGTAGCCATGTCGTTCTGTGAGCCAAGGAGTTG

What the best way to trim it? What tool better to use and what mode to use?


Thank you in advance,

Best wishes,

Alex

RNA-Seq MGISEQ adapter trimming • 972 views
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