I have a question about how
vg treats reads that are multi-mapping to different chromosomes but
are then anchored to the correct location by their mate read.
Normally, a multi-mapping read is assigned a MAPQ of 0. However, if
vg manages to rescue the pair, will it adjust the MAPQ to be above 0 for both fragments?
I know this is the case with
I just want to make sure that the following command will not discard multi-mapping reads that are anchored to the correct location by their mate:
vg filter aligned.gam -t 40 -r 0.90 -fu -m 1 -q 10 -D 999 -x index.xg > filtered.gam
Thank you in advance.