I have a question about gene size normalization.
Usually, when comparing intra-sample gene expression, we need to normalize intra-sample variation. The most common method is gene lenght correction, because longer genes will have more reads.
But I'm analyzing small RNA sequencing (5 million reads).
My question is: how should I normalize intra-sample small RNA variation? Does the same "gene lenght correction" makes sense, and if it does, should I correct for precursor size or by final product size?
Intra-sample variation is important for me because I need to know which miRNAs are most expressed in each sample