Number of junctions to be inserted on the fly is larger than the limitSjdbInsertNsj=1000000 in STARSolo

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3.2 years ago

mitrafarahmandnejad • 0

I got the following error when tried to map my scrna seq to a concatenated genome via STARSolo in usegalexy.

Fatal LIMIT error: the number of junctions to be inserted on the fly =5416911 is larger than the limitSjdbInsertNsj=1000000
Fatal LIMIT error: the number of junctions to be inserted on the fly =5416911 is larger than the limitSjdbInsertNsj=1000000
SOLUTION: re-run with at least --limitSjdbInsertNsj 5416911

Jan 26 05:12:14 ...... FATAL ERROR, exiting
Detected Common Potential Problems
The tool was executed with one or more duplicate input datasets. This frequently results in tool errors due to problematic input choices.

Does anyone know how can I increase limitSjdbInsertNsj in galaxy environment? Or any solution to get rid of this error?

RNA-Seq scrna • 1.0k views

ADD COMMENT • link updated 3.2 years ago by Ram 43k • written 3.2 years ago by mitrafarahmandnejad • 0

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I'd recommend posting in https://help.galaxyproject.org/ as that is the Galaxy specific help forum.

ADD REPLY • link 3.2 years ago by Ram 43k

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