Hello, I want to normalise my microbiome data (otu table) using ancom-bc (I want to have a csv file with data that is normalised for library size). I am doing that:
feature.table=feature_table; sample.var="sample_id"; group.var="group" zero.cut=0.90; lib.cut=1000; neg.lb=FALSE pre.process=feature_table_pre_process(feature.table, metdadata, sample.var, group.var, zero.cut, lib.cut, neg.lb) feature.table=pre.process$feature.table
Am I doing it right? is my feature.table my normalised matrix? I am asking as after feature_table_pre_process I have the same values but some of my OTUs are gone.