RNA seq analysis with TCGA
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7 months ago
leticia ▴ 20

Hello everybody , I need your help please. I downloaded TCGA breast cancer data from Cbioportal ," mRNA Expression, RSEM (Batch normalized from Illumina HiSeq_RNASeqV2)" i know that it's already normalized but which type of normalization? is it FPKM?

cbioportal normalisation RNA-Seq TCGA • 433 views
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There are lots of tutorials here and there for this job. Strongly recommend to pick one and follow accordingly for this kind of job. always it would be helpful for one to get familiarize them self with concepts before doing analysis. Anyway expression values could not be in RSEM and FPKM at the same time!!!

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