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3.1 years ago
kajol21bm
•
0
I am new to the NGS analysis field and have started learning analysis of Whole Genome Bisulphite Sequencing Data. I have two biological replicates for each of my sample. Some sample replicates have more than 5% difference in Mapping Efficiency. What difference in % mapping efficiency among the biological replicates is allowed? Also, what is the best way to proceed for further analysis: like pooling of biological replicates (in methylkit for DMR finding) or proceeding separately with each replicates and using logistic regression for DMR finding?
If you want to do any statistical analysis then you must not just merge them, use dedicatedtools such as How to do DMR analysis with BiSeq (for RRBS methylation data)?