I have a , maybe, silly question. I have downloaded table with raw counts and normalized counts that looks like this
sample_id raw_read_count gene_id normalized_read_count 168-2-1 9685 ENSG00000000003 48.56 168-2-1 25 ENSG00000000005 0.25 168-2-1 4630 ENSG00000000419 47.89 168-2-1 1847 ENSG00000000457 6.81
and other table with the metadata info
sample_id treatment 168-2-1 Pre-treatment 004-1-5 Pre-treatment 005-1-8 Pre-treatment 034-1-0 Pre-treatment 034-3-8 Post-treatment
And I was wondering how is the best way to do a DE analysis using data, as I always start from RNAseq samples, I don't have much idea about how to start. Any advice or tip would be great!! Thanks!!