I know that my question is simple but I have a problem with understanding the transcript database. In big simplification I don’t understand the strand orientation. I read that one strand is + while the other is - and it means that forward (+) strand is coding, and reverse (-) is a template. So if my gene of interest is on reverse strand, is that mean that in this case - strand is coding ? When I search a database (like for example ensembl) I have a transcript written in 5->3 direction. So can I use it to find the right primers ? Or should I have reverse complement ?