Entering edit mode
3.1 years ago
candron
▴
10
Hi All,
I am using ShortStack to identify miRNAs (and other types of sRNAs) from 5 samples of the plant Nicotiana benthamiana - 2 WT and 3 mutants. The samples are fastq files from sRNA sequencing.
Any experience on whether one should run each sample separately with ShortStack or all 5 at the same time? Documentation says that one can specify several readfiles as inputs, but I wonder what's the best strategy here.
Any information would be greatly appreciated.
Thanks in advance!