Hi,
I got PacBio Sequel data sequenced in CLR mode. I used bedtools bamtofastq on the *subreads.bam files to extract the subreads in FASTQ format. Instead of subreads I got CLR though. Using PacBio's BAM2fastx tools I was able to extract the subreads. I was under the assumption that the subreads.bam files contain subreads and not CLR. Am I wrong or is something fishy either with bamtofastq or the data?
Thanks, Chris
I have the same question. Is bedtools bam2fastq is appropriate for converting pacbio bam file to fastq format?