bamToFastq on Sequel subreads.bam
0
0
Entering edit mode
3 months ago

Hi,

I got PacBio Sequel data sequenced in CLR mode. I used bedtools bamtofastq on the *subreads.bam files to extract the subreads in FASTQ format. Instead of subreads I got CLR though. Using PacBio's BAM2fastx tools I was able to extract the subreads. I was under the assumption that the subreads.bam files contain subreads and not CLR. Am I wrong or is something fishy either with bamtofastq or the data?

Thanks, Chris

PacBio bam subreads • 175 views
ADD COMMENT

Login before adding your answer.

Traffic: 2245 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6