I'm trying to use blast agains a de novo trinity assembled transcriptome.
blastn -query /trimmed_concat_trinity_assembly.fa \ -db nt \ -outfmt 11 \ -max_target_seqs 5 \ -num_threads 24 \ -out /trimmed_concat_trinity_assembled_blastn.nt_20210203.asn
It runs for quite a while (>60 hours) before I get the following error message:
Error: (802.4) [blastn] [Cannot close serializing output stream] Exception: basic_ios::clear: iostream error BLAST failed to write output
However, the output file is present (and absolutely enormous! More than 4 x the assembled transcriptome, btu I haven't run out of space, so that doesn't seem to be it).