I dont have a locally KEGG database bought,but I want to Blast my sequences against only the KEGG part of nr db cause you know blast whole nr is really time consuming.
Here is my approach:
- download the KO number of a specific pathway that I'm interested in from the KEGG server
- convert the KO number downloaded into GI number
- then use the blast+ 's gilist function to Blast the GI numbers that I downloaded.
But MY PROBLEM is: I dont know how to do the step 1 and step 2, could anyone provide some procedure to do that? Many thanks,