I have a public scRNA dataset containing an expression matrix normalized by the deconvolution method in scran. The study sequenced two different types of cells; I am focusing on just one gene, and would like to see if it is differentially expressed between these two cell types. I am new to bioinformatics, and have only analyzed bulk RNA-seq data by DESeq2 before. I know there are similar programs for scRNA-seq, but they all seem to require raw count input, while I have a non-integer matrix. I have seen previous posts of people trying to input scran-normalized counts into MAST, and am wondering if this is recommended? Would it be bad practice to forgo the program and perform a Mann Whitney test on the normalized counts for my gene of interest instead?