Question: How To Convert Bigwig To Bam?
1
gravatar for ashis.csedu
6.9 years ago by
ashis.csedu80
United States
ashis.csedu80 wrote:

I am working with some bigwig data that were generated from bedGraph data from an RNA-seq experiment. Since the bigwig files I have list coverage scores with 1bp span. These bigwig/bedGraphs were generated from BAM files that I don't have, but can I re-create those BAM files from BigWigs? Thanks.

bam bigwig • 7.0k views
ADD COMMENTlink written 6.9 years ago by ashis.csedu80
6
gravatar for Jorge Amigo
6.9 years ago by
Jorge Amigo11k
Santiago de Compostela, Spain
Jorge Amigo11k wrote:

unfortunately not. BAM files are alignments, reads that came out from a sequencing experiment and that have been mapped to a reference sequence. bigwig and bedgraph files are just informative for coverage, number of reads for each particular site of that reference sequence, like summaries of what the original BAM file had. you can't generate the original BAM file from the coverage file: it would be as "I have this SNP allele frequency for all populations around the world, can I get all the genotypes of all the individual samples from it?". it's just a conceptual mistake.

ADD COMMENTlink written 6.9 years ago by Jorge Amigo11k

Thanks for clarifying the mis-understanding. It helps.

ADD REPLYlink written 6.9 years ago by ashis.csedu80
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