Can you average values to do WGCNA?
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9 months ago
gerastuff • 0

Hello everyone!

I was just wondering if when doing a WGCNA of many samples with lots of different experimental groups (so for instance this is about 120 different conditions, like serum/serum free, LPS, tx1, tx2 etc.) and having quadruplicates of the data, can you average each gene from its 4 replicates and construct the network from that?

I've been thinking about pro's and cons.. and can't think of a strong reason of why it shouldn't be feasible...

RNA-Seq WGCNA R Gene Network • 204 views
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Averaging means reduction of power, I do not see any reason to do that. Do you have concerns towards RAM usage or runtime?

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