This error appears if my output file is at /mnt/...(drive). But if an output file is in home directory - trinity runs well And some strange situation, when I wrote *sudo" the program cannot find salmon, but without this one, it runs well. When i run trinity i have the next error:
failed to create symbolic link 'left.norm.fq': Operation not supported Error, cmd: ln -sf /mnt/lapd/Vika_data/Denovo/trinity3/insilico_read_normalization/L3_R1_pair.fastq.normalized_K25_maxC200_minC1_maxCV10000.fq left.norm.fq died with ret 256 at /home/katya/Soft/trinityrnaseq-v2.11.0/util/insilico_read_normalization.pl line 793. Error, cmd: /home/katya/Soft/trinityrnaseq-v2.11.0/util/insilico_read_normalization.pl --seqType fq --JM 200G --max_cov 200 --min_cov 1 --CPU 50 --output /mnt/lapd/Vika_data/Denovo/trinity3/insilico_read_normalization --max_CV 10000 --left /mnt/lapd/Vika_data/Denovo/trim2/L3_R1_pair.fastq --right /mnt/lapd/Vika_data/Denovo/trim2/L3_R2_pair.fastq --pairs_together --PARALLEL_STATS died with ret 512 at /home/katya/Soft/trinityrnaseq-v2.11.0/Trinity line 2826. main::process_cmd("/home/katya/Soft/trinityrnaseq-v2.11.0/util/insilico_read_nor"...) called at /home/katya/Soft/trinityrnaseq-v2.11.0/Trinity line 3379 main::normalize("/mnt/lapd/Vika_data/Denovo/trinity3/insilico_read_normalization", 200, ARRAY(0x558a27931ee0), ARRAY(0x558a27931ec8)) called at /home/katya/Soft/trinityrnaseq-v2.11.0/Trinity line 3319 main::run_normalization(200, ARRAY(0x558a27931ee0), ARRAY(0x558a27931ec8)) called at /home/katya/Soft/trinityrnaseq-v2.11.0/Trinity line 1372
I guess the account you are running this from does not have write permissions to the mounted directory (
But when i use other programs, i don't have the same error
Is the drive filesystem NTFS or exFAT?
I am not sure, but read in google, I think its NTFS
I have the exact same issue when trinity-2.4.0 runs from vdjpuzzle- version 3.
Have you found out what was they issue?
Thank you in advance, Evi
Sorry, but I cannot help you with anything, as I have not found this answer. We just asked to add more memory to our server because the error lies in using a network drive.