Entering edit mode
3.2 years ago
anasofiamoreira94
▴
80
Good morning, I would like to develop/use an algorithm to analyze GC content of a primer and temperature melting. I'm not used to this type of analysis. I'm going to use python, thus can someone point me to an algorithm that it is already designed, for me to implement in python? Thank you so much for the help!
Primer3 and similar tools already exist, are tested and well-accepted why reinventing the wheel?
I tried with Primer3, however the TM is different than the TM presented in IDT site. I designed a python algorithm to calculate manually the tm of the primer: 64.9 + 41 *(GC - 16.4)/(GCAT), but the tm continues to be different. Do you know a way to get the same result as the IDT site?
It depends on the buffer. You should use a tool that is aware of the buffer composition of the polymerase. Almost all companies offer such tools, e.g. NEB or promega.
Can I have one open source to use with python?