Duplicates probability depending on the number of flow cells
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7 weeks ago

In this article there is such a statement about WGS reads from Illumina HiSeq 2500:

the probability of obtaining repeated reads from two independent flow cells is higher than from one cell

But I don't see how using 2 cells can result in higher rate of duplicates compared to 1 cell (in case of sequencing depth and other conditions being equal). Can anybody explain it?

next-gen sequencing duplicates • 91 views
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