Question: Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?
gravatar for giulia.trauzzi
12 days ago by
giulia.trauzzi0 wrote:

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have looked into Trimmomatic, Cutadapt (that I have used for the adapter removal) and FLEXBAR. As I have a series of barcodes used throughout my samples (720 samples), FLEXBAR seemed the only one that accepts a .fasta file with the barcodes specified. My question is if I can run the program to detect and remove the barcode sequences across my samples without going through the demultiplexing again. Thanks

ADD COMMENTlink written 12 days ago by giulia.trauzzi0
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1255 users visited in the last hour