Question: Chip_seq analysis remove duplicates
gravatar for luckysardar171
2 days ago by
luckysardar1710 wrote:

Hello everyone I am analyzing Chip-seq data. In fragmentation step we used Micrococcal Nuclease in this condition can I remove duplicate reads from bam file after mapping or not can anyone suggest me. Read many related papers some people are removing duplicates and some are not confused what to do. PCR showing bands 150, 300, 450, 600 etc. is this fragmentation size matters in chip-seq because more than 1k bands are visible as its almost cutting around random nuleosome position. I need some expert opinion.

Thank you

chip-seq • 52 views
ADD COMMENTlink modified 2 days ago • written 2 days ago by luckysardar1710

For MNase ChIP-seq I would avoid removing PCR duplicates unless you have UMIs on your adapters. Fragment ends are expected to be more uniform since you are chewing unbound DNA away instead of fragmenting in a semi-stochastic manner.

ADD REPLYlink written 2 days ago by rpolicastro3.9k

Okay, thank you for the reply, My fragment size varying lot does it matters in chip-seq

ADD REPLYlink written 2 days ago by luckysardar1710
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