Heya. I'm here to understand lanes in RNA-SEQ. I got my data from my sequencer provider with the file naming convention showing 4 lanes in total. Each experimental condition is found 3 times in each lane. The way I'm reading this is that I have 4 replicates. Am I correct?
I have been chatting here but I have been told to start a new post so here I am. Also, I think I asked the wrong question.
The file naming convention looks like this: (the first column is sample name and the second is the lane)
a1 | LANE 1 a2 | LANE 1 a3 | LANE 1 b1 | LANE 1 b2 | LANE 1 b3 | LANE 1 a1 | LANE 2 a2 | LANE 2 a3 | LANE 2 b1 | LANE 2 b2 | LANE 2 b3 | LANE 2
I have 4 lanes in total with 5 different samples ( that repeat 3 times each ) per lane - the same samples are repeating on each lane. I have also been told we have 4 replicates for each prep. So the a1, a2 and a3 from each line is from the sequencing?
From those 5 different samples: 1 is the control, 1 is the negative and the other 3 are different experimental conditions. I really have difficulties understanding the experimental design here. Any input would be useful. Thank you!