How RNAseq input file for ssGSAE should be normalized?
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9 months ago
Raheleh ▴ 210

Hi all,

I have RNAseq of 12 mouse models (4 genotypes) that are vst normalized using Deseq2. I want to do ssGSEA analysis to see the enrichment of hallmark geneset for each sample. Should they be normalized for gene length as well?

Many thanks for your help!

VST ssGSEA normalization • 393 views
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