I am looking for tools/methods for somatic variant calling for unpaired samples. The somatic variant calling through GATK best practices is specifically for tumor/normal paired samples. My study entails somatic variant analysis studying complex disorders, thus I have unpaired samples. I found following options: Samtools, ISOWN, Mutect (only tumor mode) and SomVarIUS . I would like to compile as many options as i could and choose the best suitable for my study. Kindly suggest further options and any opinions and suggestions are welcome.