Violation of Independence test for differential methylation (DM)
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6 months ago
CY ▴ 610

People say that the assumption of indenpendence is violated if using contengency table for differential methylation. How exactly does indenpendence is violated? Can anyone explain that a little bit? Thanks in advance

methylation differential methylation • 215 views
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Hi, it always depend how do you create the contingency table, but I think the idea is that neighbooring CpG positions have correlated methylation levels and simply summing them up (or testing them one by one and then applying a simple FDR correction) is not taking this dependency into account.

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Thanks for explaining. But when a single CpG site was tested, the contingency table sums up the (normalized) methylation counts of this CpG site from each sample (patient). I thought the independence we are looking for is the methylation level of this site between different samples, right?

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