DiffBind with duplicates
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3 months ago
jc2842 • 0

Hi I'm a novice for ChIP-seq and trying to use DiffBind.

I have ChIP-seq dat of WT and mutant1 for H3K27me3. Each genotype has 2 replicates.

When I try the differential analysis, I get an error message "2 groups and at least triplicates for each group is required".

I understand triplicates are kinda minimum with meaningful stats but as a preview I wonder if I can run with duplicates before I generate more replicates.

DiffBind ChIP-Seq • 148 views
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Entering edit mode
3 months ago
jc2842 • 0

got it. i need to use minMember option. thanks.

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