Excuse me, everyone, I am new in this field. I need help
There are several SRR file with same suffix, such as (GSM1169462 is sample id):
SRR914044_GSM1169462_XXX_1w_r2_Mus_musculus_RNA-Seq.fastq.gz
SRR914045_GSM1169462_XXX_1w_r2_Mus_musculus_RNA-Seq.fastq.gz
SRR914046_GSM1169462_XXX_1w_r2_Mus_musculus_RNA-Seq.fastq.gz
should I merge these file before running STAR?
And there is r1, r2 suffix in the file, should I merge r1 and r2 before running STAR? I am not sure they were biological replicate or pairwise file
Thank you!
These appear to be 5 single-end runs. And sample itself is called
paITR_1w_r2
https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SAMN02208963&o=acc_s%3AaThank you for your kindly answer. I don't understand why were this sample run 5 times? should I treat each run SRR9140** ID as a replicate sample?
it's a common (required) practice. Those are biological replicates of the experiment and are needed to do reliable statistics on the data later on.
so yes, they are to be seen as replicates (biological NOT technical replicates)