Imagine I am doing paired end illumina sequencing of this simple DNA.
5'-GTACGATCGTGCATCGATCGTACGTACGTACGTAGCGTACG-3'
3'-CGTACGCTACGTACGTACGTACGATCGATGCACGATCGTAC-5'
The first read would cover the bases similar to this (i.e. GTACGAT):
read 1
-------------->
5'-GTACGATCGTGCATCGATCGTACGTACGTACGTAGCGTACG-3'
3'-CGTACGCTACGTACGTACGTACGATCGATGCACGATCGTAC-5'
Then, the strand would flip over to neighboring oligos that are on the flow cell and the second read would be synthesized.
My question now is, if the second read covers the bases like this (i.e. GCATGC):
(please ignore the underscores, I don't know how to show white space in the code sample)
read 1 read 2
--------------> <---------
5'-GTACGATCGTGCATCGATCGTACGTACGTACGTAGCGTACG-3'
3'-CGTACGCTACGTACGTACGTACGATCGATGCACGATCGTAC-5'
Or like this (i.e. CATGCTA):
read 1
-------------->
5'-GTACGATCGTGCATCGATCGTACGTACGTACGTAGCGTACG-3'
3'-CGTACGCTACGTACGTACGTACGATCGATGCACGATCGTAC-5'
<-----------
read 2
I would appreciate a lot if somebody could clear this up for me.
Cheers!
I've cleaned up your post for you. The formatting option you were using is the block quote option, not the code formatting option. The button for the code formatting option is the
101010
button.