Question about a paper called Reference flow: reducing reference bias using multiple population genomes
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3.1 years ago

l am a starters ,l'm reading "Reference flow: reducing reference bias using multiple population genomes".Have a question about the Merging and lifting. "For reads that aligned to more than one reference, we must choose a single “best” alignment to include in the ultimate SAM output. We select by choosing the alignment with the highest alignment score; roughly, this corresponds to the alignment with the fewest mismatches and gaps. "

This "score” refers to mapq?how can l get the best alignment?

thanks for your reply.

RNA-Seq sequence • 503 views
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