How to normalise read depth in scRNA-seq data, with usegalaxy
0
0
Entering edit mode
7 weeks ago

Hello all,

I have a number of C1 fluidigm scRNA-seq data (paired-ended) using Smart-Seq2, but with different sequencing depths. Is it possible to normalise for sequencing depths in usegalaxy after using STAR to map them on the mouse genome?

Also, can one also compare single-end and paired-end data after mapping them?

Cheers,

Y

scRNA-seq read-depth • 126 views
ADD COMMENT
0
Entering edit mode

Please ask at the Galaxy forum: https://galaxyproject.org/support/

ADD REPLY

Login before adding your answer.

Traffic: 1021 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6