Nanopore data error
0
0
Entering edit mode
7 months ago
mailard • 0

Hello!

I use ONT data

I mapped reads with minimap2: -a -x splice -k 14 and etc - next use samtools to sort

But when I go to the next step to identify splice events I have the error:

SAM validation error: ERROR: Record 1, Read name 741eead6-18e3-4fb4-baa7-b2cb28ecb1aa, CIGAR covers 2466 bases but the sequence is 0 read bases

Can you explain to me why I have this error? Maybe I have a problem with my fastq file?

RNA-Seq • 231 views
ADD COMMENT
0
Entering edit mode

Please use the formatting bar (especially the code option) to present your post better. You can use backticks for inline code (`text` becomes text), or select a chunk of text and use the highlighted button to format it as a code block. If your code has long lines with a single command, break those lines into multiple lines with proper escape sequences so they're easier to read and still run when copy-pasted. I've done it for you this time.
code_formatting

ADD REPLY

Login before adding your answer.

Traffic: 2979 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6