Nanopore data error
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7 months ago
mailard • 0

Hello!

I use ONT data

I mapped reads with minimap2: -a -x splice -k 14 and etc - next use samtools to sort

But when I go to the next step to identify splice events I have the error:

SAM validation error: ERROR: Record 1, Read name 741eead6-18e3-4fb4-baa7-b2cb28ecb1aa, CIGAR covers 2466 bases but the sequence is 0 read bases


Can you explain to me why I have this error? Maybe I have a problem with my fastq file?

RNA-Seq • 231 views
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