Nanopore data error

Question

[mailard]

Hello!

I use ONT data

I mapped reads with minimap2: -a -x splice -k 14 and etc - next use samtools to sort

But when I go to the next step to identify splice events I have the error:

SAM validation error: ERROR: Record 1, Read name 741eead6-18e3-4fb4-baa7-b2cb28ecb1aa, CIGAR covers 2466 bases but the sequence is 0 read bases

Can you explain to me why I have this error? Maybe I have a problem with my fastq file?

[Ram]

Please use the formatting bar (especially the code option) to present your post better. You can use backticks for inline code (`text` becomes text), or select a chunk of text and use the highlighted button to format it as a code block. If your code has long lines with a single command, break those lines into multiple lines with proper escape sequences so they're easier to read and still run when copy-pasted. I've done it for you this time.