I have result of RNA sequencing FastQC Report.
It was failed in Per base sequence content, but passed Adapter Content.
I went to https://sequencing.qcfail.com/articles/positional-sequence-bias-in-random-primed-libraries/.
It seems the primers problems.
How I can fix this?
Thanks in advance for great help!
Appears that my answer from yesterday was lost during the cut-over to the new biostars codebase. Basically, there is nothing to fix here as the linked blog post concludes. The data should align well.
Thank you so much for your great again!
it could not be a problem, the recent library preparation uses a transposase system that generates that pattern on the first bases, check what kit was used.
Thank you so much for your great help!
Thank you again!
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