Entering edit mode
11.7 years ago
Richard
▴
590
Hopefully an easy one for blast users...
My command: blastn.exe -query query1.fa -subject subject.fa -outfmt 5
My best hit ( as determined by the lowest expectation value ) contains the following:
CCCGGCCAGGCCCCCAGACTGCTGATCTAC-G-G-C-GC--CAGGGCCACCGGCATCCCC
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CCCGGCCAGGCCCCCAGACTGCTGATCTACGGCGCCAGCAGCAGGGCCACCGGCATCCCC
where my query is on the top. Perhaps you can spot what the question will be already. Although this alignment isn't incorrect, there is an alternate solution that contains just one 6 base deletion. Can anyone point me to a parameter I can use to get just one larger deletion in place of many small deletions?