Blast Indel Calibration
1
0
Entering edit mode
11.7 years ago
Richard ▴ 590

Hopefully an easy one for blast users...

My command: blastn.exe -query query1.fa -subject subject.fa -outfmt 5

My best hit ( as determined by the lowest expectation value ) contains the following:

CCCGGCCAGGCCCCCAGACTGCTGATCTAC-G-G-C-GC--CAGGGCCACCGGCATCCCC
|||||||||||||||||||||||||||||| | | | ||  |||||||||||||||||||
CCCGGCCAGGCCCCCAGACTGCTGATCTACGGCGCCAGCAGCAGGGCCACCGGCATCCCC

where my query is on the top. Perhaps you can spot what the question will be already. Although this alignment isn't incorrect, there is an alternate solution that contains just one 6 base deletion. Can anyone point me to a parameter I can use to get just one larger deletion in place of many small deletions?

blastn • 2.6k views
ADD COMMENT
3
Entering edit mode
11.7 years ago
Richard ▴ 590

Looks like all I needed was "-task blastn". By default it uses megablast.

ADD COMMENT

Login before adding your answer.

Traffic: 1606 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6