I am having some problems running tblastx locally on my HPC. I built my own database and I am trying to run tblastx on a small data set (to then move into larger analyses) but I am getting an error (I am using blast 2.2.26)
This is my command to run tblastx:
$ tblastx -num_threads 8 -query nucleotide.fasta -db nucleotide_db -out tblastx_out.xml -evalue 1e-10 -outfmt 5
And this is the error:
Error: NCBI C++ Exception: "/../blast/2.2.26/src/ncbi-blast-2.2.26+-src/c++/src/objects/seq/../seqalign/Seq_align.cpp", line 210: Error: CSeq_align::GetSeqRange(): Row is empty
I used basically the same command with blastp (of course changing the query and database) and it worked fine.
Any help would be much appreciated.