Question: Cuffdiff Fpkm Values
2
gravatar for KS
6.6 years ago by
KS360
KS360 wrote:

Hello everyone,

I am comparing two samples (control and treated) paired end RNA Seq data in Galaxy. In the cuffdiff output I have noticed that few genes have zero FPKM value in one sample and other sample has significant FPKM value. I want to identify uniquely expressed genes identified only in one sample (either control or treated).

My question: If something is measured with significance in one sample (high FPKM), but not measured at all in another sample (Zero FPKM), should I consider that gene as significant?

Thanks

suz

galaxy cuffdiff rna-seq • 3.4k views
ADD COMMENTlink written 6.6 years ago by KS360
1
gravatar for JC
6.6 years ago by
JC7.7k
Mexico
JC7.7k wrote:

Yes, but also check for the differential expression significance. More information: http://cufflinks.cbcb.umd.edu/howitworks.html#hdif

ADD COMMENTlink written 6.6 years ago by JC7.7k
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