Dear users, I have a question regarding microarray data analysis (Affymetrix one color). My point is that I have just 1 sample TREATMENT and 1 sample REFERENCE. Neither technical replicates nor biological replicates are available. A statistical test to find differentially expressed genes between the two conditions seems to me impossible (even the simple t-test) due to the absence of replicates. People who asked me to do the analysis were interested only in finding the genes changing between the two conditions. In this conditions, in my opinion only the fold change is possible just to give a general view of the behavior of the genes. Any other suggestion about this issue?
Thanks a lot