Hi every one,

I am so happy to find you as I am new in NGS area. I have a set of miRNA data coming from Solid sequencer. I want to do the differential expression analysis of data. In fact, I have two groups and have 1 library per group without replicate. I want to know which statistical model fit better with my experiment Have any idea, please tell me! I have already looked at the library edger and DESeq Thanks Sara

I don't think a lack of replicates lends itself well to any statistical model I'm afraid.

What was wrong with the approaches provided by DESeq and EdgeR?

I am also wondering what's wrong with the model of negative binomial distribution implemented in edgeR. I once used it to analyze RNA-seq data with only one replicate for each treatment. There is a discussion on SEQanswers in which you may be interested: http://seqanswers.com/forums/showthread.php?t=3951