Entering edit mode
12.0 years ago
litiancheng.gansu
▴
10
I want to blast my 36SE reads to a special region of HG19, does NCBI BLAST can do this special region search? If so, how can I do this?
for example, I only want my reads to be blasted to chr1:5000-10000 of hg19, how can I do this without creating a special small region?
Yes, I can create a small region sequence database, but I don't like to because I have to convert the relative coordinate to hg19 coordinate. You may ask me why not covert relative coordinate to hg19 coordinate by add a offset, but I insist on blasting regionly because I have soon many targets regions and I have to uniform all coordinate to detect something.