What Does The Term "Steady State Mrna" Mean?
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9.0 years ago

Hi,

This question might be really stupid, but I'm a beginner and information on this would help a lot: I would like to know what exactly people mean when they refer to "steady state mRNA". Could someone help please?

Thanks, Rama

mrna • 13k views
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9.0 years ago
JC 12k

from this paper:

We assume that the change of mRNA level (dR/dt) depends linearly on mRNA synthesis and degradation,

dR/dt = txj[DNA] - dj[RNAj]

where [RNAj] is the RNA concentration for gene j, [DNA] is constant ([DNA]=1), txj is the transcription rate, and dj is the degradation rate of gene j. For simplification, we initially assume the degradation rate to be constant, meaning independent of gene j. Therefore in steady state where dR/dt=0, the RNA concentration of gene j is proportional to transcription and degradation rates of gene j.

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Hey JC, thanks for the help. As I said, I am completely new to the field, and this is extremely helpful for me. I am clear on what the term means, plus how it is obtained as well. Thank you, once again!

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Also, I read that RNAseq is used to sequence steady state mRNA. Is there an indicator/procedure to figure out when a cell's mRNA is in steady state?

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Should've accepted this as the right answer way back when - it would seem I abandoned this profile and now when I was trying to create a dummy profile, resurrected this by accident. Apologies!

-@RamRS

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