Question: Trinity - Type Of Reads: ( Cfa, Cfq, Fa, Or Fq )
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gravatar for Biomonika (Noolean)
7.8 years ago by
State College, PA, USA
Biomonika (Noolean)3.1k wrote:

Hi there!

I accidentaly ran Trinity with cfq parameter instead of fq which I believe is what I wanted - fastq with quality scores. I would really like to know what cfq stands for as I tried to google it with no success :/

from Trinity help: --seqType <string> :type of reads: ( cfa, cfq, fa, or fq )

Thanks for suggestions.

trinity • 2.5k views
ADD COMMENTlink modified 7.8 years ago by Farhat2.9k • written 7.8 years ago by Biomonika (Noolean)3.1k
3
gravatar for Farhat
7.8 years ago by
Farhat2.9k
Pune, India
Farhat2.9k wrote:

It stands for colorspace fastq, the output from a SOLiD sequencer. For a brief primer check http://marketing.appliedbiosystems.com/images/Product_Microsites/Solid_Knowledge_MS/pdf/CSHL_Fu.pdf

ADD COMMENTlink modified 7.8 years ago • written 7.8 years ago by Farhat2.9k

Thank you. I had Illumina data, but all with really high quality scores. Can I expect that results will be similiar if I ran it with cfq instead of fq? (Will compare both runs though)

ADD REPLYlink modified 7.8 years ago • written 7.8 years ago by Biomonika (Noolean)3.1k
2

No, it will be completely nonsensical most likely.

ADD REPLYlink written 7.8 years ago by Farhat2.9k
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