I am trying to analyse a set of metagenome sequences using MEGAN upon which I am facing some issues. I created a local BLAST database in my system that contains 16S rRNA sequences. I ran a blastn against this database and got the output. When I tried to input the file into MEGAN through the import from blast option, I encounter the below error.
java.io.IOException: Line 1: ';' expected, got: 's'
Meanwhile I tried running a blastx against the nr database for the same set of sequences, which I was able to analyse through MEGAN successfully .I want to understand whats actually causing this error. Is this a problem with the local database that I have created and is there something else that needs to be addressed??
Kindly help me out with this.