I have a very simple question!! Actually I have just started working with Mev, it's amazing and user friendly. But the problem is I dont know much about statistics, I mean I am a biology man! I have uploaded my data on MeV, it seems that I should do some normalization, etc. I have 7 samples (time line) with 3 replications, what shall I do with replication, should I just consider one of them as expression data or ? Here is the information of my experiment :
Human Genome U133 Plus 2.0 Array 40000 genes = 40000 row 7 sample (time) * 3 rep = 24 column and
a part of data : every three of them are for one sample/time (replication for one experiment )
AFFX-BIOB-5_AT **7.81507 7.773 8.3** ,.....