Can i consider an sequence to be full-length cDNA, if the ORF in the sequence aligns with ~99%-100% identity, with 100% coverage to the reference protein? The species i work on does not have any nearest reference.
Could i please know, if there are any tools/scripts available which can run locally to predict full-length cDNA sequences. I know tools like TargetIdentifier and Full-lengther which can run online, but they eat a lot of time (for running BlastX). (edit: bit confused with target identifier terminology (eg: sense complete and partial), full-lengther does not work any more)
I would also like to appreciate if someone guides me in this issue. Pleae spare if i am not able to put properly.
Thanks in advance.