I have a Illumnia generated fasta file that was created using rpoB sequence data. I am looking to BLAST these sequences in order to find the best 3 hits of what organism I am looking at. I know you can do something similar to this in QIIME, using BLASTALL, but with a 16S reference database.
Does anyone know of a rpoB database or a way that I could accurately identify the sequences in my FASTA file?
Any help or advice would be greatly appreciated.
Best Regards, Paul